저출력레이저가 성인의 치은섬유아세포의 성장양상과 미세구조에 미치는 영향에 관한 실험적 연구
An Experimental Study on Growth pattern and Ultrastructure of Human Gingival Fibroblasts Treated by Low level Laser
안낙현, 오윤상,
소속 상세정보
안낙현 ( ) - 전남대학교
오윤상 ( ) - 전북대학교
KMID : 0355219920170020129
Abstract
In order to verify the acceleration effect of low level laser(LLL) on oral mucosal wound healing process at cell biological level, the author studied growth pattern and ultrastructure in human gingival fibroblasts flashed by GaAlAs LLL-830nm,
15mW-for
10 minutes/flash one to three times at interval of 3-4 days through the evaluation of cell growth rate, protein conent/cell, DNA content/cell and ultrastructural changes for 14 days.
@ES The results were as follows;
@EN 1. The growth rate in gingival fibroblasts treated by LLL showed 4 orderly stages-decreasing stage after LLL treatment, acute increasing stage 3 days after LLL treatment, resting stage and recovering stage.
2. The effect of multiple flashes of LLL at interval of 3 days more or less was not proportional to times of flash on acceleration of growth in gingival fibroblasts.
3. The total protein content per gingival fibroblast was not significantly changed by LLL treatment in comparison with control group. but some kinds of protein which might be cell growth promoting factors were decreased immediately after LLL
treatment,
thereafter were acutely increased in cellular protein profile.
4. In ultrastructural changes of gingival fibroblasts treated by LLL, more prominent rough endoptasmic reticulum, mitochondrial hyperplasia/hypertrophy and increased extracellular fibrillar matrix were observed in comparison with control group
under
same experimental period.
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